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Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

We discovered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR product amplified with Pfu DNA polymerase. A high molecular mass (>250 kDa) complex with PCR-enhancing activity was purified from Pfu extracts. The complex is a multimer of two discrete...

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Autors principals: Hogrefe, Holly H., Hansen, Connie J., Scott, Bradley R., Nielson, Kirk B.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC117351/
https://ncbi.nlm.nih.gov/pubmed/11782527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.012372799
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