Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation
We discovered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR product amplified with Pfu DNA polymerase. A high molecular mass (>250 kDa) complex with PCR-enhancing activity was purified from Pfu extracts. The complex is a multimer of two discrete pro...
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| Yayımlandı: | Proc Natl Acad Sci U S A |
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| Asıl Yazarlar: | , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
National Academy of Sciences
2002
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117351/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11782527/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.012372799 |
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