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Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation
We discovered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR product amplified with Pfu DNA polymerase. A high molecular mass (>250 kDa) complex with PCR-enhancing activity was purified from Pfu extracts. The complex is a multimer of two discrete...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
The National Academy of Sciences
2002
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC117351/ https://ncbi.nlm.nih.gov/pubmed/11782527 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.012372799 |
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