Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

We discovered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR product amplified with Pfu DNA polymerase. A high molecular mass (>250 kDa) complex with PCR-enhancing activity was purified from Pfu extracts. The complex is a multimer of two discrete...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Hogrefe, Holly H., Hansen, Connie J., Scott, Bradley R., Nielson, Kirk B.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: The National Academy of Sciences 2002
Pynciau:
Biological Sciences
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC117351/
https://ncbi.nlm.nih.gov/pubmed/11782527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.012372799
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