Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

We discovered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR product amplified with Pfu DNA polymerase. A high molecular mass (>250 kDa) complex with PCR-enhancing activity was purified from Pfu extracts. The complex is a multimer of two discrete pro...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Hogrefe, Holly H., Hansen, Connie J., Scott, Bradley R., Nielson, Kirk B.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2002
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117351/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11782527/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.012372799
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