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Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

We discovered a thermostable enzyme from the archaeon Pyrococcus furiosus (Pfu), which increases yields of PCR product amplified with Pfu DNA polymerase. A high molecular mass (>250 kDa) complex with PCR-enhancing activity was purified from Pfu extracts. The complex is a multimer of two discrete pro...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Hogrefe, Holly H., Hansen, Connie J., Scott, Bradley R., Nielson, Kirk B.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2002
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117351/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11782527/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.012372799
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