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Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis
To develop a simple, speedy, economical and widely applicable method for multiple-site mutagenesis, we have substantially modified the Quik-Change™ Site-Directed Mutagenesis Kit protocol (Stratagene, La Jolla, CA). Our new protocol consists of (i) a PCR reaction using an in vitro technique, LDA (lig...
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Main Authors: | , |
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Format: | Artigo |
Sprog: | Inglês |
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Oxford University Press
2000
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Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC108465/ https://ncbi.nlm.nih.gov/pubmed/10931937 |
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