Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis
To develop a simple, speedy, economical and widely applicable method for multiple-site mutagenesis, we have substantially modified the Quik-Change™ Site-Directed Mutagenesis Kit protocol (Stratagene, La Jolla, CA). Our new protocol consists of (i) a PCR reaction using an in vitro technique, LDA (lig...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2000
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC108465/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10931937/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.16.e78 |
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