Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis

To develop a simple, speedy, economical and widely applicable method for multiple-site mutagenesis, we have substantially modified the Quik-Change™ Site-Directed Mutagenesis Kit protocol (Stratagene, La Jolla, CA). Our new protocol consists of (i) a PCR reaction using an in vitro technique, LDA (lig...

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Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Sawano, Asako, Miyawaki, Atsushi
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 2000
Предметы:	NAR Methods Online
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC108465/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10931937/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.16.e78
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