Engineering and Characterization of 3-Aminotyrosine-Derived Red Fluorescent Variants of Circularly Permutated Green Fluorescent Protein

Introducing 3-aminotyrosine (aY), a noncanonical amino acid (ncAA), into green fluorescent protein (GFP)-like chromophores shows promise for achieving red-shifted fluorescence. However, inconsistent results, including undesired green fluorescent species, hinder the effectiveness of this approach. In...

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Главные авторы:	Hao Zhang, Xiaodong Tian, Jing Zhang, Hui-wang Ai
Формат:	Artigo
Язык:	Inglês
Опубликовано:	MDPI AG 2024-01-01
Серии:	Biosensors
Предметы:	nonconical amino acid protein engineering redox sensitivity red-shifted fluorescence
Online-ссылка:	https://www.mdpi.com/2079-6374/14/1/54
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