Engineering and Characterization of 3-Aminotyrosine-Derived Red Fluorescent Variants of Circularly Permutated Green Fluorescent Protein

Introducing 3-aminotyrosine (aY), a noncanonical amino acid (ncAA), into green fluorescent protein (GFP)-like chromophores shows promise for achieving red-shifted fluorescence. However, inconsistent results, including undesired green fluorescent species, hinder the effectiveness of this approach. In...

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Ngā kaituhi matua:	Hao Zhang, Xiaodong Tian, Jing Zhang, Hui-wang Ai
Hōputu:	Artigo
Reo:	Inglês
I whakaputaina:	MDPI AG 2024-01-01
Rangatū:	Biosensors
Ngā marau:	nonconical amino acid protein engineering redox sensitivity red-shifted fluorescence
Urunga tuihono:	https://www.mdpi.com/2079-6374/14/1/54
Ngā Tūtohu:	Tāpirihia he Tūtohu Kāore He Tūtohu, Me noho koe te mea tuatahi ki te tūtohu i tēnei pūkete!