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Circularly permuted green fluorescent proteins engineered to sense Ca(2+)

To visualize Ca(2+)-dependent protein–protein interactions in living cells by fluorescence readouts, we used a circularly permuted green fluorescent protein (cpGFP), in which the amino and carboxyl portions had been interchanged and reconnected by a short spacer between the original termini. The cpG...

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Detalhes bibliográficos
Main Authors: Nagai, Takeharu, Sawano, Asako, Park, Eun Sun, Miyawaki, Atsushi
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC30630/
https://ncbi.nlm.nih.gov/pubmed/11248055
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.051636098
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