Circularly permuted green fluorescent proteins engineered to sense Ca(2+)

To visualize Ca(2+)-dependent protein–protein interactions in living cells by fluorescence readouts, we used a circularly permuted green fluorescent protein (cpGFP), in which the amino and carboxyl portions had been interchanged and reconnected by a short spacer between the original termini. The cpG...

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Bibliografische Detailangaben

Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Nagai, Takeharu, Sawano, Asako, Park, Eun Sun, Miyawaki, Atsushi
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 2001
Schlagworte:	Biological Sciences
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC30630/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11248055/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.051636098
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