Circularly permuted green fluorescent proteins engineered to sense Ca(2+)
To visualize Ca(2+)-dependent protein–protein interactions in living cells by fluorescence readouts, we used a circularly permuted green fluorescent protein (cpGFP), in which the amino and carboxyl portions had been interchanged and reconnected by a short spacer between the original termini. The cpG...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC30630/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11248055/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.051636098 |
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