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Circularly permuted green fluorescent proteins engineered to sense Ca(2+)
To visualize Ca(2+)-dependent protein–protein interactions in living cells by fluorescence readouts, we used a circularly permuted green fluorescent protein (cpGFP), in which the amino and carboxyl portions had been interchanged and reconnected by a short spacer between the original termini. The cpG...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The National Academy of Sciences
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC30630/ https://ncbi.nlm.nih.gov/pubmed/11248055 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.051636098 |
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