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Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state

N.BstNBI is a nicking endonuclease that recognizes the sequence GAGTC and nicks one DNA strand specifically. The Type IIs endonuclease, MlyI, also recognizes GAGTC, but cleaves both DNA strands. Sequence comparisons revealed significant similarities between N.BstNBI and MlyI. Previous studies showed...

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Bibliografische gegevens
Hoofdauteurs: Besnier, Caroline E., Kong, Huimin
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2001
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1084030/
https://ncbi.nlm.nih.gov/pubmed/11520857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/embo-reports/kve175
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