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Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state

N.BstNBI is a nicking endonuclease that recognizes the sequence GAGTC and nicks one DNA strand specifically. The Type IIs endonuclease, MlyI, also recognizes GAGTC, but cleaves both DNA strands. Sequence comparisons revealed significant similarities between N.BstNBI and MlyI. Previous studies showed...

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Hlavní autoři: Besnier, Caroline E., Kong, Huimin
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2001
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1084030/
https://ncbi.nlm.nih.gov/pubmed/11520857
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/embo-reports/kve175
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