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Engineering a nicking endonuclease N.AlwI by domain swapping
Changing enzymatic function through genetic engineering still presents a challenge to molecular biologists. Here we present an example in which changing the oligomerization state of an enzyme changes its function. Type IIs restriction endonucleases such as AlwI usually fold into two separate domains...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
The National Academy of Sciences
2001
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC60812/ https://ncbi.nlm.nih.gov/pubmed/11687651 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.241215698 |
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