Engineering a nicking endonuclease N.AlwI by domain swapping
Changing enzymatic function through genetic engineering still presents a challenge to molecular biologists. Here we present an example in which changing the oligomerization state of an enzyme changes its function. Type IIs restriction endonucleases such as AlwI usually fold into two separate domains...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC60812/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11687651/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.241215698 |
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