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A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease

A stable heterodimeric protein containing a single correctly folded catalytic domain (SCD) of T7 endonuclease I was produced by means of a trans-splicing intein system. As predicted by a model presented earlier, purified SCD protein acts a non-specific nicking endonuclease on normal linear DNA. The...

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Bibliografiska uppgifter
Huvudupphovsmän: Guan, Chudi, Kumar, Sanjay
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2005
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1277808/
https://ncbi.nlm.nih.gov/pubmed/16264086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki921
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