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Catalytic and binding mutants of the junction-resolving enzyme endonuclease I of bacteriophage t7: role of acidic residues.

Endonuclease I is a 149 amino acid protein of bacteriophage T7 that is a Holliday junction-resolving enzyme, i.e. a four-way junction-selective nuclease. We have performed a systematic mutagenesis study of this protein, whereby all acidic amino acids have been individually replaced by other residues...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Parkinson, M J, Pöhler, J R, Lilley, D M
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1999
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC148232/
https://ncbi.nlm.nih.gov/pubmed/9862997
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