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Catalytic and binding mutants of the junction-resolving enzyme endonuclease I of bacteriophage t7: role of acidic residues.

Endonuclease I is a 149 amino acid protein of bacteriophage T7 that is a Holliday junction-resolving enzyme, i.e. a four-way junction-selective nuclease. We have performed a systematic mutagenesis study of this protein, whereby all acidic amino acids have been individually replaced by other residues...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Principais autores: Parkinson, M J, Pöhler, J R, Lilley, D M
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC148232/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9862997/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/27.2.682
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