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A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
A sequence-specific nicking endonuclease from Streptomyces designated as DC13 was purified to near homogeneity. Starting with 30 grams of wet cells, the enzyme was purified by ammonium sulfate fractionation, DEAE cellulose, and phenyl-Sepharose chromatography. The purified protein had a specific act...
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| Publicado no: | ISRN Biochem |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Hindawi Publishing Corporation
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4392989/ https://ncbi.nlm.nih.gov/pubmed/25937959 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2013/287158 |
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