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A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties

A sequence-specific nicking endonuclease from Streptomyces designated as DC13 was purified to near homogeneity. Starting with 30 grams of wet cells, the enzyme was purified by ammonium sulfate fractionation, DEAE cellulose, and phenyl-Sepharose chromatography. The purified protein had a specific act...

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Détails bibliographiques
Publié dans:ISRN Biochem
Auteurs principaux: Somyoonsap, Peechapack, Kitpreechavanich, Vichein, Pornbanlualap, Somchai
Format: Artigo
Langue:Inglês
Publié: Hindawi Publishing Corporation 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4392989/
https://ncbi.nlm.nih.gov/pubmed/25937959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2013/287158
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