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Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases

We describe a new approach for labeling of unique sequences within dsDNA under nondenaturing conditions. The method is based on the site-specific formation of vicinal nicks, which are created by nicking endonucleases (NEases) at specified DNA sites on the same strand within dsDNA. The oligomeric seg...

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Sonraí Bibleagrafaíochta
Main Authors: Kuhn, Heiko, Frank-Kamenetskii, Maxim D.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Oxford University Press 2008
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373456/
https://ncbi.nlm.nih.gov/pubmed/18344522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn107
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