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Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases
We describe a new approach for labeling of unique sequences within dsDNA under nondenaturing conditions. The method is based on the site-specific formation of vicinal nicks, which are created by nicking endonucleases (NEases) at specified DNA sites on the same strand within dsDNA. The oligomeric seg...
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
2008
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2373456/ https://ncbi.nlm.nih.gov/pubmed/18344522 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn107 |
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