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Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state
N.BstNBI is a nicking endonuclease that recognizes the sequence GAGTC and nicks one DNA strand specifically. The Type IIs endonuclease, MlyI, also recognizes GAGTC, but cleaves both DNA strands. Sequence comparisons revealed significant similarities between N.BstNBI and MlyI. Previous studies showed...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2001
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1084030/ https://ncbi.nlm.nih.gov/pubmed/11520857 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/embo-reports/kve175 |
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