Expression and Purification of the Recombinant Lethal Factor of Bacillus anthracis

The structural gene for the 90-kDa lethal factor (LF) isolated from Bacillus anthracis was expressed as a fusion protein with six histidine residues in Escherichia coli. Expression of LF in E. coli under the transcriptional regulation of the T5 promoter yielded a soluble cytosolic protein with an ap...

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Vydáno v:	Infect Immun
Hlavní autoři:	Gupta, Pankaj, Batra, Smriti, Chopra, Arun P., Singh, Yogendra, Bhatnagar, Rakesh
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1998
Témata:	Molecular and Cellular Pathogenesis
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107985/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9453657/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.66.2.862-865.1998
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