Expression and Purification of the Recombinant Lethal Factor of Bacillus anthracis

The structural gene for the 90-kDa lethal factor (LF) isolated from Bacillus anthracis was expressed as a fusion protein with six histidine residues in Escherichia coli. Expression of LF in E. coli under the transcriptional regulation of the T5 promoter yielded a soluble cytosolic protein with an ap...

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Hlavní autoři: Gupta, Pankaj, Batra, Smriti, Chopra, Arun P., Singh, Yogendra, Bhatnagar, Rakesh
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 1998
Témata:
Molecular and Cellular Pathogenesis
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC107985/
https://ncbi.nlm.nih.gov/pubmed/9453657
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