Expression and Purification of the Recombinant Lethal Factor of Bacillus anthracis
The structural gene for the 90-kDa lethal factor (LF) isolated from Bacillus anthracis was expressed as a fusion protein with six histidine residues in Escherichia coli. Expression of LF in E. coli under the transcriptional regulation of the T5 promoter yielded a soluble cytosolic protein with an ap...
Guardat en:
| Publicat a: | Infect Immun |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1998
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107985/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9453657/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.66.2.862-865.1998 |
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