Cofactor Engineering: a Novel Approach to Metabolic Engineering in Lactococcus lactis by Controlled Expression of NADH Oxidase

NADH oxidase-overproducing Lactococcus lactis strains were constructed by cloning the Streptococcus mutans nox-2 gene, which encodes the H(2)O-forming NADH oxidase, on the plasmid vector pNZ8020 under the control of the L. lactis nisA promoter. This engineered system allowed a nisin-controlled 150-f...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Lopez de Felipe, Felix, Kleerebezem, Michiel, de Vos, Willem M., Hugenholtz, Jeroen
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1998
Sujets:	Physiology and Metabolism
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC107362/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9683475/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.180.15.3804-3808.1998
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