Scintillation Proximity Assay for Inhibitors of Escherichia coli MurG and, Optionally, MraY

MurG and MraY, essential enzymes involved in the synthesis of bacterial peptidoglycan, are difficult to assay because the substrates are lipidic and hard to prepare in large quantities. Based on the use of Escherichia coli membranes lacking PBP1b, we report a high-throughput method to measure the ac...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Ravishankar, Sudha, Kumar, Vidya Prasanna, Chandrakala, B., Jha, Ramesh K., Solapure, Suresh M., de Sousa, Sunita M.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2005
Pynciau:
Mechanisms of Action: Physiological Effects
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1068599/
https://ncbi.nlm.nih.gov/pubmed/15793120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.49.4.1410-1418.2005
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