Fluorescence Detection-Based Functional Assay for High-Throughput Screening for MraY

We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using UDP-MurNAc-N(ɛ)-dansylpentapeptide, a fluorescent derivative of the MraY nucleotide substrate, and a partially purified p...

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Detalhes bibliográficos
Main Authors: Stachyra, Thérèse, Dini, Christophe, Ferrari, Paul, Bouhss, Ahmed, van Heijenoort, Jean, Mengin-Lecreulx, Dominique, Blanot, Didier, Biton, Jacques, Le Beller, Dominique
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Mechanisms of Action: Physiological Effects
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC353143/
https://ncbi.nlm.nih.gov/pubmed/14982781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.48.3.897-902.2004
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