Fluorescence Detection-Based Functional Assay for High-Throughput Screening for MraY

We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using UDP-MurNAc-N(ɛ)-dansylpentapeptide, a fluorescent derivative of the MraY nucleotide substrate, and a partially purified p...

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Detalhes bibliográficos

Publicado no:	Antimicrob Agents Chemother
Principais autores:	Stachyra, Thérèse, Dini, Christophe, Ferrari, Paul, Bouhss, Ahmed, van Heijenoort, Jean, Mengin-Lecreulx, Dominique, Blanot, Didier, Biton, Jacques, Le Beller, Dominique
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2004
Assuntos:	Mechanisms of Action: Physiological Effects
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC353143/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14982781/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AAC.48.3.897-902.2004
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