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Fluorescence Detection-Based Functional Assay for High-Throughput Screening for MraY
We have developed a novel assay specific to MraY, which catalyzes the first membrane step in the biosynthesis of bacterial cell wall peptidoglycan. This was accomplished by using UDP-MurNAc-N(ɛ)-dansylpentapeptide, a fluorescent derivative of the MraY nucleotide substrate, and a partially purified p...
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| 主要な著者: | , , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology
2004
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC353143/ https://ncbi.nlm.nih.gov/pubmed/14982781 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.48.3.897-902.2004 |
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