Scintillation Proximity Assay for Inhibitors of Escherichia coli MurG and, Optionally, MraY

MurG and MraY, essential enzymes involved in the synthesis of bacterial peptidoglycan, are difficult to assay because the substrates are lipidic and hard to prepare in large quantities. Based on the use of Escherichia coli membranes lacking PBP1b, we report a high-throughput method to measure the ac...

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Bibliographic Details
Main Authors: Ravishankar, Sudha, Kumar, Vidya Prasanna, Chandrakala, B., Jha, Ramesh K., Solapure, Suresh M., de Sousa, Sunita M.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2005
Subjects:
Mechanisms of Action: Physiological Effects
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1068599/
https://ncbi.nlm.nih.gov/pubmed/15793120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.49.4.1410-1418.2005
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