Scintillation Proximity Assay for Inhibitors of Escherichia coli MurG and, Optionally, MraY

MurG and MraY, essential enzymes involved in the synthesis of bacterial peptidoglycan, are difficult to assay because the substrates are lipidic and hard to prepare in large quantities. Based on the use of Escherichia coli membranes lacking PBP1b, we report a high-throughput method to measure the ac...

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Detaylı Bibliyografya
Asıl Yazarlar: Ravishankar, Sudha, Kumar, Vidya Prasanna, Chandrakala, B., Jha, Ramesh K., Solapure, Suresh M., de Sousa, Sunita M.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology 2005
Konular:
Mechanisms of Action: Physiological Effects
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1068599/
https://ncbi.nlm.nih.gov/pubmed/15793120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.49.4.1410-1418.2005
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