Scintillation Proximity Assay for Inhibitors of Escherichia coli MurG and, Optionally, MraY

MurG and MraY, essential enzymes involved in the synthesis of bacterial peptidoglycan, are difficult to assay because the substrates are lipidic and hard to prepare in large quantities. Based on the use of Escherichia coli membranes lacking PBP1b, we report a high-throughput method to measure the ac...

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Main Authors: Ravishankar, Sudha, Kumar, Vidya Prasanna, Chandrakala, B., Jha, Ramesh K., Solapure, Suresh M., de Sousa, Sunita M.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2005
Assuntos:
Mechanisms of Action: Physiological Effects
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1068599/
https://ncbi.nlm.nih.gov/pubmed/15793120
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.49.4.1410-1418.2005
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