PCR-Based DNA Amplification and Presumptive Detection of Escherichia coli O157:H7 with an Internal Fluorogenic Probe and the 5′ Nuclease (TaqMan) Assay

Presumptive identification of Escherichia coli O157:H7 is possible in an individual, nonmultiplexed PCR if the reaction targets the enterohemorrhagic E. coli (EHEC) eaeA gene. In this report, we describe the development and evaluation of the sensitivity and specificity of a PCR-based 5′ nuclease ass...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Oberst, R. D., Hays, M. P., Bohra, L. K., Phebus, R. K., Yamashiro, C. T., Paszko-Kolva, C., Flood, S. J. A., Sargeant, J. M., Gillespie, J. R.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9726887/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.9.3389-3396.1998
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