PCR-Based DNA Amplification and Presumptive Detection of Escherichia coli O157:H7 with an Internal Fluorogenic Probe and the 5′ Nuclease (TaqMan) Assay

Presumptive identification of Escherichia coli O157:H7 is possible in an individual, nonmultiplexed PCR if the reaction targets the enterohemorrhagic E. coli (EHEC) eaeA gene. In this report, we describe the development and evaluation of the sensitivity and specificity of a PCR-based 5′ nuclease ass...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Oberst, R. D., Hays, M. P., Bohra, L. K., Phebus, R. K., Yamashiro, C. T., Paszko-Kolva, C., Flood, S. J. A., Sargeant, J. M., Gillespie, J. R.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1998
Schlagworte:	Methods
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9726887/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.9.3389-3396.1998
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