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A 5′ Nuclease PCR (TaqMan) High-Throughput Assay for Detection of the mecA Gene in Staphylococci

In an effort to find a rapid, efficient, and reliable method of screening large numbers of bacterial isolates for specific antimicrobial resistance genes, we compared conventional PCR results to the results generated using the TaqMan 5′ nuclease PCR kit in conjunction with an ABI Prism 7700 Sequence...

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Detalhes bibliográficos
Main Authors: Killgore, George E., Holloway, Brian, Tenover, Fred C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC86956/
https://ncbi.nlm.nih.gov/pubmed/10878035
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