PCR-Based DNA Amplification and Presumptive Detection of Escherichia coli O157:H7 with an Internal Fluorogenic Probe and the 5′ Nuclease (TaqMan) Assay

Presumptive identification of Escherichia coli O157:H7 is possible in an individual, nonmultiplexed PCR if the reaction targets the enterohemorrhagic E. coli (EHEC) eaeA gene. In this report, we describe the development and evaluation of the sensitivity and specificity of a PCR-based 5′ nuclease ass...

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書誌詳細
主要な著者: Oberst, R. D., Hays, M. P., Bohra, L. K., Phebus, R. K., Yamashiro, C. T., Paszko-Kolva, C., Flood, S. J. A., Sargeant, J. M., Gillespie, J. R.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology 1998
主題:
Methods
オンライン･アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC106737/
https://ncbi.nlm.nih.gov/pubmed/9726887
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