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Strategy for Optimizing DNA Amplification in a Peripheral Blood PCR Assay Used for Diagnosis of Human Brucellosis

We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by...

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Bibliografische gegevens
Hoofdauteurs: Morata, Pilar, Queipo-Ortuño, Maria Isabel, de Dios Colmenero, Juan
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 1998
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC105141/
https://ncbi.nlm.nih.gov/pubmed/9705371
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