Strategy for Optimizing DNA Amplification in a Peripheral Blood PCR Assay Used for Diagnosis of Human Brucellosis

We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Morata, Pilar, Queipo-Ortuño, Maria Isabel, de Dios Colmenero, Juan
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Bacteriology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105141/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9705371/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.9.2443-2446.1998
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