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Development and Evaluation of a PCR-Enzyme-Linked Immunosorbent Assay for Diagnosis of Human Brucellosis

In order to overcome some of the limitations of conventional microbiological techniques in the diagnosis of human brucellosis, a simple PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed. After amplification of a 223-bp sequence of a gene that codes for the synthesis of an immunogenetic...

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Autori principali: Morata, Pilar, Queipo-Ortuño, María I., Reguera, Jose M., García-Ordoñez, Miguel A., Cárdenas, Ana, Colmenero, Juan D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2003
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC149602/
https://ncbi.nlm.nih.gov/pubmed/12517839
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.1.144-148.2003
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