Development and Evaluation of a PCR-Enzyme-Linked Immunosorbent Assay for Diagnosis of Human Brucellosis

In order to overcome some of the limitations of conventional microbiological techniques in the diagnosis of human brucellosis, a simple PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed. After amplification of a 223-bp sequence of a gene that codes for the synthesis of an immunogenetic...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Morata, Pilar, Queipo-Ortuño, María I., Reguera, Jose M., García-Ordoñez, Miguel A., Cárdenas, Ana, Colmenero, Juan D.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2003
Assuntos:	Bacteriology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC149602/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12517839/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.1.144-148.2003
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