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Strategy for Optimizing DNA Amplification in a Peripheral Blood PCR Assay Used for Diagnosis of Human Brucellosis

We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by...

תיאור מלא

שמור ב:
הוצא לאור ב:J Clin Microbiol
Principais autores: Morata, Pilar, Queipo-Ortuño, Maria Isabel, de Dios Colmenero, Juan
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology (ASM) 1998
נושאים:
Bacteriology
גישה מקוונת:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105141/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9705371/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.9.2443-2446.1998
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