Strategy for Optimizing DNA Amplification in a Peripheral Blood PCR Assay Used for Diagnosis of Human Brucellosis

We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by...

Täydet tiedot

Bibliografiset tiedot

Julkaisussa:	J Clin Microbiol
Päätekijät:	Morata, Pilar, Queipo-Ortuño, Maria Isabel, de Dios Colmenero, Juan
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	American Society for Microbiology (ASM) 1998
Aiheet:	Bacteriology
Linkit:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC105141/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9705371/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.9.2443-2446.1998
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