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Preparation of partially (2)H/(13)C-labelled RNA for NMR studies. Stereo-specific deuteration of the H5′′ in nucleotides

An effective in vitro enzymatic synthesis is described for the production of nucleoside triphosphates (NTPs) which are stereo-specifically deuterated on the H5′′ position with high selectivity (>98%), and which can have a variety of different labels ((13)C, (15)N, (2)H) in other positions. The NT...

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Autori principali: Cromsigt, Jenny, Schleucher, Jürgen, Gustafsson, Tomas, Kihlberg, Jan, Wijmenga, Sybren
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC101849/
https://ncbi.nlm.nih.gov/pubmed/11917025
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