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Preparation of partially (2)H/(13)C-labelled RNA for NMR studies. Stereo-specific deuteration of the H5′′ in nucleotides
An effective in vitro enzymatic synthesis is described for the production of nucleoside triphosphates (NTPs) which are stereo-specifically deuterated on the H5′′ position with high selectivity (>98%), and which can have a variety of different labels ((13)C, (15)N, (2)H) in other positions. The NT...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
2002
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC101849/ https://ncbi.nlm.nih.gov/pubmed/11917025 |
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