Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus

The CRISPR-Cas9 system has the potential to revolutionize genome editing in human pluripotent stem cells (hPSCs), but its advantages and pitfalls are still poorly understood. We systematically tested the ability of CRISPR-Cas9 to mediate reporter gene knockin at 16 distinct genomic sites in hPSCs. W...

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Huvudupphovsmän: Florian T. Merkle, Werner M. Neuhausser, David Santos, Eivind Valen, James A. Gagnon, Kristi Maas, Jackson Sandoe, Alexander F. Schier, Kevin Eggan
Materialtyp: Artigo
Språk:Inglês
Publicerad: Elsevier 2015-05-01
Serie:Cell Reports
Länkar:http://www.sciencedirect.com/science/article/pii/S2211124715003824
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