Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus

Por Florian T. Merkle, Werner M. Neuhausser, David Santos, Eivind Valen, James A. Gagnon, Kristi Maas, Jackson Sandoe, Alexander F. Schier, Kevin Eggan
Publicado em 2015-05-01

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.

Por James A Gagnon, Eivind Valen, Summer B Thyme, Peng Huang, Laila Akhmetova, Andrea Pauli, Tessa G Montague, Steven Zimmerman, Constance Richter, Alexander F Schier
Publicado em 2014-01-01