Streamlined high-throughput cloning protocol to generate arrayed mutant libraries
Summary: Large-scale, site-directed mutagenesis enables rapid characterization of the biochemical and biological properties of proteins. Here, we present a cost-effective and adaptable cloning pipeline to generate arrayed gene libraries for a construct of interest. We detail steps to use an open acc...
שמור ב:
| Principais autores: | , , , , , , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Elsevier
2023-03-01
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| סדרה: | STAR Protocols |
| נושאים: | |
| גישה מקוונת: | http://www.sciencedirect.com/science/article/pii/S2666166722008103 |
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