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Streamlined high-throughput cloning protocol to generate arrayed mutant libraries

Summary: Large-scale, site-directed mutagenesis enables rapid characterization of the biochemical and biological properties of proteins. Here, we present a cost-effective and adaptable cloning pipeline to generate arrayed gene libraries for a construct of interest. We detail steps to use an open acc...

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Guardado en:
Autores principales: Kerry T. Sun, Tark S. Patel, Justin Kim, Helen S.H. Tang, Ghazaleh Eskandari-Sedighi, Haresh Sureshkumar, Dean Schieve, S.A. Mok
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier 2023-03-01
Colección:STAR Protocols
Materias:
Genetics
Sequencing
High Throughput Screening
Molecular Biology
Acceso en línea:http://www.sciencedirect.com/science/article/pii/S2666166722008103
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