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Metabolic Labeling of Yeast RNA with Radioactive Uracil

To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from total RNA. Therefore, metabolic labeling is necessary t...

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Sábháilte in:
Sonraí bibleagrafaíochta
Príomhchruthaitheoir: Yuehua Wei
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Bio-protocol LLC 2013-04-01
Sraith:Bio-Protocol
Rochtain ar líne:https://bio-protocol.org/en/bpdetail?id=623&type=0
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